Alfacalcidol enhances collagen quality in ovariectomized rat bones
Identifieur interne : 002048 ( Main/Exploration ); précédent : 002047; suivant : 002049Alfacalcidol enhances collagen quality in ovariectomized rat bones
Auteurs : Hideaki Nagaoka ; Masahiko Terajima ; Shizuka Yamada [Japon] ; Yoshiaki Azuma [Japon] ; Takayuki Chida [Japon] ; Mitsuo Yamauchi [États-Unis]Source :
- Journal of Orthopaedic Research [ 0736-0266 ] ; 2014-08.
Abstract
The aim of this study was to investigate the effects of alfacalcidol (1α(OH)D3: ALF) on bone collagen employing an ovariectomized rat model. Thirty‐five 16‐week‐old female Sprague‐Dawley rats were divided into five groups: SHAM (sham‐operated + vehicle), OVX (ovariectomy + vehicle), and three ALF‐treated groups, that is, ovariectomy + 0.022 µg/kg/day ALF, ovariectomy + 0.067 µg/kg/day ALF, and ovariectomy + 0.2 µg/kg/day ALF. After 12 weeks of treatment, tibiae were subjected to histological, biochemical and immunohistochemical analyses. Collagen matrices in OVX bone appeared as immature and poorly organized; however, with the ALF treatment, it was improved in a dose‐dependent manner. Contents of collagen and pyridinoline cross‐link were decreased in OVX compared with SHAM, but they increased to the level comparable to SHAM in ALF‐treated groups. The total aldehyde, that is, a sum of free and those involved cross‐links, in the highest dose of ALF was significantly higher than the rest of the groups (p < 0.05). In addition, the expression of lysyl oxidase was increased in the all ALF‐treated groups compared with OVX (p < 0.05). In conclusion, ALF increases not only the amount of collagen but also enhances the maturation of collagen in ovariectomy‐induced osteoporotic bones, which likely contributes to the improvement of bone quality. © 2014 The Authors. Journal of Orthopaedic Research. Published by Wiley Periodicals, Inc. J Orthop Res 32:1030–1036, 2014.
Url:
DOI: 10.1002/jor.22642
Affiliations:
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xml:lang="en">The aim of this study was to investigate the effects of alfacalcidol (1α(OH)D3: ALF) on bone collagen employing an ovariectomized rat model. Thirty‐five 16‐week‐old female Sprague‐Dawley rats were divided into five groups: SHAM (sham‐operated + vehicle), OVX (ovariectomy + vehicle), and three ALF‐treated groups, that is, ovariectomy + 0.022 µg/kg/day ALF, ovariectomy + 0.067 µg/kg/day ALF, and ovariectomy + 0.2 µg/kg/day ALF. After 12 weeks of treatment, tibiae were subjected to histological, biochemical and immunohistochemical analyses. Collagen matrices in OVX bone appeared as immature and poorly organized; however, with the ALF treatment, it was improved in a dose‐dependent manner. Contents of collagen and pyridinoline cross‐link were decreased in OVX compared with SHAM, but they increased to the level comparable to SHAM in ALF‐treated groups. The total aldehyde, that is, a sum of free and those involved cross‐links, in the highest dose of ALF was significantly higher than the rest of the groups (p < 0.05). In addition, the expression of lysyl oxidase was increased in the all ALF‐treated groups compared with OVX (p < 0.05). In conclusion, ALF increases not only the amount of collagen but also enhances the maturation of collagen in ovariectomy‐induced osteoporotic bones, which likely contributes to the improvement of bone quality. © 2014 The Authors. Journal of Orthopaedic Research. Published by Wiley Periodicals, Inc. J Orthop Res 32:1030–1036, 2014.</div></front></TEI><affiliations><list><country><li>Japon</li><li>États-Unis</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Nagaoka, Hideaki" sort="Nagaoka, Hideaki" uniqKey="Nagaoka H" first="Hideaki" last="Nagaoka">Hideaki Nagaoka</name><name sortKey="Terajima, Masahiko" sort="Terajima, Masahiko" uniqKey="Terajima M" first="Masahiko" last="Terajima">Masahiko Terajima</name></noCountry><country name="Japon"><noRegion><name sortKey="Yamada, Shizuka" sort="Yamada, Shizuka" uniqKey="Yamada S" first="Shizuka" last="Yamada">Shizuka Yamada</name></noRegion><name sortKey="Azuma, Yoshiaki" sort="Azuma, Yoshiaki" uniqKey="Azuma Y" first="Yoshiaki" last="Azuma">Yoshiaki Azuma</name><name sortKey="Chida, Takayuki" sort="Chida, Takayuki" uniqKey="Chida T" first="Takayuki" last="Chida">Takayuki Chida</name></country><country name="États-Unis"><noRegion><name sortKey="Yamauchi, Mitsuo" sort="Yamauchi, Mitsuo" uniqKey="Yamauchi M" first="Mitsuo" last="Yamauchi">Mitsuo Yamauchi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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